Journal: Nature Communications

Article Title: Microfluidics for understanding model organisms

doi: 10.1038/s41467-022-30814-6

Figure Lengend Snippet: Novel microfabrication techniques combined with creativity enable the production of different microfluidic tools that, in turn, facilitate the development of novel experimental paradigms to be used with small multicellular model organisms. Microfluidics empower researchers with tremendous control over sample stimulation while also enabling precise manipulation of samples, and being compatible with high-resolution live imaging. The potential of these microtechnologies is accelerating as they are integrated with additional technologies, become more accessible, and are designed to be multifunctional. Figure created with BioRender.com.

Article Snippet: Fig. 3 Microfluidic systems can deliver stimulations with high precision and at throughputs not previously attainable while making once laborious experiments less demanding. a - i A 3D-printed microfluidic device compatible with a custom-built light-sheet microscope to stimulate zebrafish with precise flow vectors for brain-wide calcium imaging . a - ii A microfluidic device with an array of microposts for analysis of sleep behavior of C. elegans . b A microfluidic system that automatically aligned Drosophila embryos and precisely compressed them using pneumatically actuated deformable sidewalls with simultaneous live imaging . c A microfluidic system capable of trapping hundreds of C. elegans embryos quickly and enabling efficient reagent exchange . d - i A microfluidic system visualized the response of olfactory receptor neurons (ORNs) of Drosophila larva in response to controlled odorant exposure . d - ii A microfluidic device with integrated glass capillaries and a microneedle for chemical injection of Drosophila larvae . e A microfluidic device exposed Drosophila embryo to a thermal gradient along the anterior-posterior axis using two laminar flow streams with different temperatures .

Techniques: Control, Imaging

Journal: Nature Communications

Article Title: Microfluidics for understanding model organisms

doi: 10.1038/s41467-022-30814-6

Figure Lengend Snippet: a-i A microfluidic device with a shape memory alloy actuator immobilized zebrafish and examined the hydrodynamic flow resultant from tail beating . a - ii A worm chamber for visualization and periodic immobilization of C. elegans . b A microfluidic device coupled with a live detection system designed to gently immobilize, orient, and inject zebrafish larvae . c - i A microfluidic system to sort fruit fly samples at high throughput and enrichment ratio . c - ii A non-invasive zebrafish larvae sorting system based on microfluidics that utilized light and acoustics to corral individual samples . All panels are cropped and adapted versions of the originals. Panel c - ii was reproduced from Mani and Chen , with the permission of AIP Publishing.

Article Snippet: Fig. 3 Microfluidic systems can deliver stimulations with high precision and at throughputs not previously attainable while making once laborious experiments less demanding. a - i A 3D-printed microfluidic device compatible with a custom-built light-sheet microscope to stimulate zebrafish with precise flow vectors for brain-wide calcium imaging . a - ii A microfluidic device with an array of microposts for analysis of sleep behavior of C. elegans . b A microfluidic system that automatically aligned Drosophila embryos and precisely compressed them using pneumatically actuated deformable sidewalls with simultaneous live imaging . c A microfluidic system capable of trapping hundreds of C. elegans embryos quickly and enabling efficient reagent exchange . d - i A microfluidic system visualized the response of olfactory receptor neurons (ORNs) of Drosophila larva in response to controlled odorant exposure . d - ii A microfluidic device with integrated glass capillaries and a microneedle for chemical injection of Drosophila larvae . e A microfluidic device exposed Drosophila embryo to a thermal gradient along the anterior-posterior axis using two laminar flow streams with different temperatures .

Techniques: High Throughput Screening Assay

Journal: Nature Communications

Article Title: Microfluidics for understanding model organisms

doi: 10.1038/s41467-022-30814-6

Figure Lengend Snippet: a - i A 3D-printed microfluidic device compatible with a custom-built light-sheet microscope to stimulate zebrafish with precise flow vectors for brain-wide calcium imaging . a - ii A microfluidic device with an array of microposts for analysis of sleep behavior of C. elegans . b A microfluidic system that automatically aligned Drosophila embryos and precisely compressed them using pneumatically actuated deformable sidewalls with simultaneous live imaging . c A microfluidic system capable of trapping hundreds of C. elegans embryos quickly and enabling efficient reagent exchange . d - i A microfluidic system visualized the response of olfactory receptor neurons (ORNs) of Drosophila larva in response to controlled odorant exposure . d - ii A microfluidic device with integrated glass capillaries and a microneedle for chemical injection of Drosophila larvae . e A microfluidic device exposed Drosophila embryo to a thermal gradient along the anterior-posterior axis using two laminar flow streams with different temperatures . All panels are cropped and adapted versions of the originals. Panel c is adapted with permission from Charles et al. , copyright 2020 American Chemical Society.

Article Snippet: Fig. 3 Microfluidic systems can deliver stimulations with high precision and at throughputs not previously attainable while making once laborious experiments less demanding. a - i A 3D-printed microfluidic device compatible with a custom-built light-sheet microscope to stimulate zebrafish with precise flow vectors for brain-wide calcium imaging . a - ii A microfluidic device with an array of microposts for analysis of sleep behavior of C. elegans . b A microfluidic system that automatically aligned Drosophila embryos and precisely compressed them using pneumatically actuated deformable sidewalls with simultaneous live imaging . c A microfluidic system capable of trapping hundreds of C. elegans embryos quickly and enabling efficient reagent exchange . d - i A microfluidic system visualized the response of olfactory receptor neurons (ORNs) of Drosophila larva in response to controlled odorant exposure . d - ii A microfluidic device with integrated glass capillaries and a microneedle for chemical injection of Drosophila larvae . e A microfluidic device exposed Drosophila embryo to a thermal gradient along the anterior-posterior axis using two laminar flow streams with different temperatures .

Techniques: Microscopy, Imaging, Injection